KOLF2.1J
The cell line is not validated yet.
WTSIi018-B-12
General
Cell Line |
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| hPSCreg name | WTSIi018-B-12 |
| Cite as: | WTSIi018-B-12 (RRID:CVCL_B5P3) |
| Alternative name(s) |
KOLF2.1J
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 24th March 2025 |
| Notes | KOLF2.1J is a well-characterized clonal iPSC line recognized for its genomic stability, high efficiency in gene editing, and robust differentiation capacity, making it a preferred model for large-scale stem cell engineering initiatives like iNDI. |
| User feedback | |
Provider |
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| Generator |
Jackson Laboratories (JAX)
Contact:
Jackson Laboratories (JAX) |
| Owner | Jackson Laboratories (JAX) |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
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| Cellosaurus | CVCL_B5P3 |
| Wikidata | Q117704576 |
| BioSamples | SAMEA117845807 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
For internal research use only. Available to academic institutions, non-profits, for-profit, and commercial organizations. |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Life Technologies |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
WTSIi018-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | KOLF2.1J is a clonal iPSC line derived from KOLF2-C1 (WTSIi018-B-1) with a corrected ARID2 variant. |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFlex™ Medium
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Transcriptome Characterisation
Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
3
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Genetic Modification
| Genetic modifications not related to a disease |
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