KOLF2.1J
WTSIi018-B-12
General
Donor Information
General Donor Information |
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Sex | male |
Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA2398402 |
HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
Is there an MTA available for the cell line? | Yes |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Life Technologies |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
WTSIi018-B.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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Selection criteria for clones | KOLF2.1J is a clonal iPSC line derived from KOLF2-C1 (WTSIi018-B-1) with a corrected ARID2 variant. |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: StemFlex™ Medium
Main protein source: Serum concentration: % |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
TRA 1-60 |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Transcriptome Characterisation
Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
3
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Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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Genetic Modification
Genetic modifications not related to a disease |
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