BNH1_iPSC_FHS1_cr_well7_JR
The cell line is not validated yet.
UMGWi004-B-1
General
Cell Line |
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| hPSCreg name | UMGWi004-B-1 |
| Cite as: | UMGWi004-B-1 |
| Alternative name(s) |
BNH1_iPSC_FHS1_cr_well7_JR
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 15th January 2025 |
| User feedback | |
Provider |
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| Generator | Department of Functional Genomics - Human Molecular Genetics (UMGW) |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA115884924 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has son:
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External Databases (Donor) |
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| BioSamples | SAMEA115116692 |
Ethics
Also have a look at the ethics information for the parental line
UMGWi004-B
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UMGWi004-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Notes on reprogramming vector detection | PCR for EBNA1 and OriP showed no residual vector in the iPSC |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | Sanger sequencing of target gene and analysis of chromatograms with online tools TIDE (http://shinyapps.datacurators.nl/tide/) and indigo (https://www.gear-genomics.com/indigo/) |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
No |
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| Passage method | Mechanically | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
mTeSR™ 1
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
Morphology
Protocol or reference
In vitro spontaneous differentiation
Morphology
Protocol or reference
In vitro spontaneous differentiation
Morphology
Protocol or reference
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46/XY
Passage number: 13
Karyotyping method:
low-coverage whole genome sequencing (lcWGS)
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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