Z1 K3v3

General

Cell Line

hPSCreg name UMGWi001-B
Cite as:
UMGWi001-B (RRID:CVCL_A1XF)
Alternative name(s)
Z1 K3v3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UMGWi004-A
(BNH1 _iPSC_WT_8)
UMGWi004-B
(BNH1 _iPSC_WT_48)
UMILi012-A
(WVS02)
Donor diseases:
Weaver Syndrome
UMILi014-A
(WVS04)
Donor diseases:
Weaver Syndrome
UMILi020-A
(WBS04)
Donor diseases:
Williams-Beuren syndrome
UMILi022-A
(WBS01)
Donor diseases:
Williams-Beuren syndrome
WTSIi167-A
(HPSI1013i-funy_1)
Donor diseases:
Bardet-Biedl syndrome
VUi005-A
(787.3)
Donor diseases:
Schizophrenia
VUi006-A
(SCZ 21.5)
Donor diseases:
Schizophrenia
VUi008-A
(SCZ 6.2)
Donor diseases:
Schizophrenia
VUi009-A
(SCZ 10.1)
Donor diseases:
Schizophrenia
VUi010-A
(SCZ 1.1)
Donor diseases:
Schizophrenia
VUi012-A
(SCZ 7.2)
Donor diseases:
Schizophrenia
VUi013-A
(804.3)
Donor diseases:
Schizophrenia
WTSIi447-A
(HPSI0416i-zige_5)
Donor diseases:
Usher syndrome
VUi017-A
(SCZ4.3)
Donor diseases:
Schizophrenia
WTSIi450-A
(HPSI0216i-aiid_1)
Donor diseases:
Usher syndrome
WTSIi452-A
(HPSI0115i-xaka_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi414-B
(HPSI0614i-eisk_6)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-B
(HPSI0614i-fovu_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi458-B
(HPSI0614i-lipl_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi573-A
(HPSI0115i-qaqp_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi576-A
(HPSI0216i-feht_1)
Donor diseases:
Usher syndrome
WTSIi402-B
(HPSI0714i-ouzk_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi465-B
(HPSI0714i-xiry_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi407-B
(HPSI0914i-hidy_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi452-B
(HPSI0115i-xaka_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi573-B
(HPSI0115i-qaqp_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi450-B
(HPSI0216i-aiid_6)
Donor diseases:
Usher syndrome
WTSIi516-B
(HPSI0216i-iogu_6)
Donor diseases:
Usher syndrome
WTSIi447-B
(HPSI0416i-zige_2)
Donor diseases:
Usher syndrome
WTSIi522-B
(HPSI0516i-pews_2)
Donor diseases:
Usher syndrome
WTSIi402-A
(HPSI0714i-ouzk_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi403-A
(HPSI0913i-rufg_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-A
(HPSI0614i-fovu_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi406-A
(HPSI0614i-mesr_4)
Donor diseases:
Bardet-Biedl syndrome
STBCi110-A
(SFC116-03-01)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
WTSIi407-A
(HPSI0914i-hidy_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi454-B
(HPSI0516i-iool_4)
Donor diseases:
Usher syndrome
WTSIi414-A
(HPSI0614i-eisk_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi147-A
(HPSI1013i-mijn_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi148-A
(HPSI0114i-veui_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi151-A
(HPSI0414i-fixm_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi152-A
(HPSI0414i-eafb_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi154-A
(HPSI0414i-lavd_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi158-A
(HPSI0914i-fuhz_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi159-A
(HPSI0714i-gibe_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi161-A
(HPSI0714i-rajk_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi165-A
(HPSI0414i-reng_4)
Donor diseases:
Bardet-Biedl syndrome
Last update 12th October 2020
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Department of Functional Genomics - Human Molecular Genetics (UMGW)
Owner Department of Functional Genomics - Human Molecular Genetics (UMGW)

External Databases

BioSamples SAMEA7222557
Cellosaurus CVCL_A1XF
Wikidata Q105511626

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • FLHS
Genetic variants
16p11.2
c.7330C>T
p.Arg2444*
Heterozygous
heterozygous mutation confirmed by Sanger sequencing

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA7211864

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. SensID GmbH, Schillingallee 68, 18057 Rostock
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information SensID GmbH, Schillingallee 68, 18057 Rostock
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of the University of Greifswald
Approval number BB047/14
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of the University of Greifswald
Approval number BB047/14
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.
Source cell origin
The soft tissue surrounding the neck of individual teeth as well as covering the alveolar bone. The tissue is fibrous and continuous with the periodontal ligament and mucosal covering.
Synonyms
  • Gingival
  • GINGIVA
  • gingiva
  • gums
  • Gum, NOS
  • Gingiva
  • Gum
  • gum
  • gum tissue
show more synonyms
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Mechanically
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
LIN28A
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
abnormal
Passage number: 2
Karyotyping method: low coverage whole genome sequencing followed by copy number variation analysis

Other Genotyping (Cell Line)