Z1 K3v3

General#

Cell Line

hPSCreg Name UMGWi001-B
Alternative name(s)
Z1 K3v3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th October 2020
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Provider

Generator Department of Functional Genomics - Human Molecular Genetics (UMGW)
Owner Department of Functional Genomics - Human Molecular Genetics (UMGW)

External Databases

BioSamples SAMEA7222557
CLO CLO_0102938

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Floating-Harbor syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • FLHS
Genetic variants
16p11.2
c.7330C>T
p.Arg2444*
Heterozygous
heterozygous mutation confirmed by Sanger sequencing

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7211864

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: SensID GmbH, Schillingallee 68, 18057 Rostock
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink SensID GmbH, Schillingallee 68, 18057 Rostock
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of the University of Greifswald
Approval number BB047/14
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of the University of Greifswald
Approval number BB047/14
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
fibroblast
A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.
Source cell origin
Gingiva
The soft tissue surrounding the neck of individual teeth as well as covering the alveolar bone. The tissue is fibrous and continuous with the periodontal ligament and mucosal covering.
Synonyms
  • Gingival
  • GINGIVA
  • gingiva
  • gums
  • Gum, NOS
  • Gingiva
  • Gum
show more synonyms
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Mechanically
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
LIN28A
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
abnormal
Passage number: 2
Karyotyping method: low coverage whole genome sequencing followed by copy number variation analysis

Other Genotyping (Cell Line)