WVS04

The cell line is not validated yet.

General

Cell Line

hPSCreg name UMILi014-A
Cite as:
UMILi014-A (RRID:CVCL_YC77)
Alternative name(s)
WVS04
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UMILi012-A
(WVS02)
Donor diseases:
Weaver Syndrome
UMILi013-A
(WVS01)
Donor diseases:
Weaver Syndrome
UMILi015-A
(WVS03)
Donor diseases:
Weaver Syndrome
UMILi025-A
(CTL09)
UMGWi001-B
(Z1 K3v3)
Donor diseases:
Floating-Harbor syndrome
UMILi020-A
(WBS04)
Donor diseases:
Williams-Beuren syndrome
UMILi022-A
(WBS01)
Donor diseases:
Williams-Beuren syndrome
WTSIi414-B
(HPSI0614i-eisk_6)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-B
(HPSI0614i-fovu_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi458-B
(HPSI0614i-lipl_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi573-A
(HPSI0115i-qaqp_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi576-A
(HPSI0216i-feht_1)
Donor diseases:
Usher syndrome
WTSIi402-B
(HPSI0714i-ouzk_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi465-B
(HPSI0714i-xiry_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi407-B
(HPSI0914i-hidy_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi452-B
(HPSI0115i-xaka_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi573-B
(HPSI0115i-qaqp_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi450-B
(HPSI0216i-aiid_6)
Donor diseases:
Usher syndrome
WTSIi516-B
(HPSI0216i-iogu_6)
Donor diseases:
Usher syndrome
WTSIi447-B
(HPSI0416i-zige_2)
Donor diseases:
Usher syndrome
WTSIi522-B
(HPSI0516i-pews_2)
Donor diseases:
Usher syndrome
WTSIi402-A
(HPSI0714i-ouzk_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi403-A
(HPSI0913i-rufg_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-A
(HPSI0614i-fovu_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi406-A
(HPSI0614i-mesr_4)
Donor diseases:
Bardet-Biedl syndrome
STBCi110-A
(SFC116-03-01)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
WTSIi407-A
(HPSI0914i-hidy_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi454-B
(HPSI0516i-iool_4)
Donor diseases:
Usher syndrome
WTSIi414-A
(HPSI0614i-eisk_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi147-A
(HPSI1013i-mijn_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi148-A
(HPSI0114i-veui_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi151-A
(HPSI0414i-fixm_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi152-A
(HPSI0414i-eafb_1)
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Bardet-Biedl syndrome
WTSIi154-A
(HPSI0414i-lavd_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi158-A
(HPSI0914i-fuhz_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi159-A
(HPSI0714i-gibe_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi161-A
(HPSI0714i-rajk_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi165-A
(HPSI0414i-reng_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi167-A
(HPSI1013i-funy_1)
Donor diseases:
Bardet-Biedl syndrome
VUi005-A
(787.3)
Donor diseases:
Schizophrenia
VUi006-A
(SCZ 21.5)
Donor diseases:
Schizophrenia
VUi008-A
(SCZ 6.2)
Donor diseases:
Schizophrenia
VUi009-A
(SCZ 10.1)
Donor diseases:
Schizophrenia
VUi010-A
(SCZ 1.1)
Donor diseases:
Schizophrenia
VUi012-A
(SCZ 7.2)
Donor diseases:
Schizophrenia
VUi013-A
(804.3)
Donor diseases:
Schizophrenia
WTSIi447-A
(HPSI0416i-zige_5)
Donor diseases:
Usher syndrome
VUi017-A
(SCZ4.3)
Donor diseases:
Schizophrenia
WTSIi450-A
(HPSI0216i-aiid_1)
Donor diseases:
Usher syndrome
WTSIi452-A
(HPSI0115i-xaka_2)
Donor diseases:
Bardet-Biedl syndrome
Last update 17th February 2023
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Provider

Generator University of Milan (UMIL)

External Databases

BioSamples SAMEA6049766
Cellosaurus CVCL_YC77
Wikidata Q98134180

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Weaver Syndrome. EED,c. 1458 C>T pHis258Tyr.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Weaver Syndrome
  • WVS
  • Weaver-Smith Syndrome

Donor Relations

All cell lines of this donor's relatives
Has father:

External Databases (Donor)

BioSamples SAMEA6049767

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: William T. Gibson https://www.bcchr.ca/wgibson
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.

Reprogramming method

Vector type Non-integrating
Vector Simplicon RNA Reprogramming Kit (Millipore)

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium TeSR™ E8™
Supplements
Pen/Strep 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)