SFC116-03-01

General

Cell Line

hPSCreg name STBCi110-A
Cite as:
STBCi110-A (RRID:CVCL_RD80)
Alternative name(s)
SFC116-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UNEWi026-A
(SF116 clone 1)
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CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi026-B
(SF116 clone 2)
Donor's gene variants:
CFH
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type 2 diabetes mellitus
age-related macular degeneration
UNEWi026-C
(SF116 clone K)
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CFH
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type 2 diabetes mellitus
age-related macular degeneration
UNEWi022-A
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CFH
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UNEWi022-B
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CFH
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age-related macular degeneration
UNEWi022-C
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CFH
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UNEWi024-A
(F180-1)
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CFH
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age-related macular degeneration
UNEWi024-B
(F180-2)
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CFH
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age-related macular degeneration
UNEWi024-C
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SCTCi014-A-1
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STBCi098-A
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STBCi100-A
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STBCi122-A
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RIi013-A
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CIRMi390-A
(CW70174)
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type 2 diabetes mellitus
CIRMi391-A
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type 2 diabetes mellitus
CIRMi400-A
(CW70080)
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type 2 diabetes mellitus
CIRMi434-A
(CW70230)
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type 2 diabetes mellitus
SCTCi012-A
(IPS19-00027)
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CIRMi452-A
(CW70255)
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SCTCi013-A
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CIRMi454-A
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Last update 29th August 2022
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104616143
EBiSC STBCi110-A
Cellosaurus CVCL_RD80
Wikidata Q54970253

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Sporadic typical T2D
Synonyms
  • NIDDM
  • non-insulin-dependent diabetes mellitus
  • type 2 diabetes
  • type II diabetes mellitus
  • insulin resistance
show more synonyms
Genetic variants
Donor is at high risk of developing age-related macular degeneration but has not been diagnosed with AMD at time of biopsy.
The donor is a carrier of a disease-associated mutation and not affected.
Synonyms
  • AMD
  • ARMD
  • Senile macular degeneration
  • Senile macular retinal degeneration
  • age related Maculopathies
  • age related maculopathy
  • age-related macular degeneration
  • macular degeneration, age-related
  • age related macular degeneration
show more synonyms
Genetic variants
CFH (target)
1q31.3
NM_000186.3:c.1204C>T
NP_000177.2:p.His402Tyr NC_000001.11:g.19669010
Homozygous
SCV000038294.5

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4582266

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information zameel.cader@ndcn.ox.ac.uk
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? NRES Committee South Central - Hampshire A
Approval number 13/SC/0179
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? NRES Committee South Central - Hampshire A
Approval number 13/SC/0179
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: DNA sequencing can only be performed for research into donors specified condition. Material shall not be sold, transplanted into any human being or used to create egg or sperm cells (gametes) or embryos. The material shall not be used for direct exploitation. For the purposes of this, Direct exploitation means to develop for commericalization or to commercialize the Material.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 8

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Passage number: 8
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)