IPS19-00051 Crispr clone H6
SCTCi015-A-1
General
Cell Line |
|
| hPSCreg name | SCTCi015-A-1 |
| Cite as: | SCTCi015-A-1 (RRID:CVCL_C0FS) |
| Alternative name(s) |
IPS19-00051 Crispr clone H6
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 2nd December 2021 |
| User feedback | |
Provider |
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| Generator | Radboudumc Stem Cell Technology Center (SCTC) |
| Owner | Radboudumc - Department of Ophthalmology |
| Distributors | |
| Derivation country | Netherlands |
External Databases |
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| BioSamples | SAMEA11350641 |
| Cellosaurus | CVCL_C0FS |
| Wikidata | Q112930353 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
CNV analysis
no mutations found |
External Databases (Donor) |
|
| BioSamples | SAMEA11350642 |
Ethics
Also have a look at the ethics information for the parental line
SCTCi015-A
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Synthego |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | not for commercial and clinical use |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
SCTCi015-A.
|
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| Passage number reprogrammed | 10 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | The clones were picked manually with a capillary pipette and a stereomicroscope |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™ Flex
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
25
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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