SCTCi015-A-1

IPS19-00051 Crispr clone H6

General#

Cell Line

hPSCreg Name
SCTCi015-A-1
Alternative name(s)
IPS19-00051 Crispr clone H6
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd December 2021
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Provider

Generator Radboudumc Stem Cell Technology Center (SCTC)
Owner Radboudumc - Department of Ophthalmology
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA11350641

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Age-Related Macular Degeneration
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • ARMD
  • AMD
  • Age-Related Macular Degeneration
  • age-related macular degeneration
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
CNV analysis
no mutations found

External Databases (Donor)

BioSamples SAMEA11350642

Ethics#

Also have a look at the ethics information for the parental line SCTCi015-A .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Synthego
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution not for commercial and clinical use

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SCTCi015-A.
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Selection criteria for clones The clones were picked manually with a capillary pipette and a stereomicroscope
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
HAND1
No
Desmin
Yes
UBERON_0000924
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
NCAM1
Yes
NESTIN
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 25

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Age-Related Macular Degeneration
Synonyms
  • ARMD
  • AMD
  • Age-Related Macular Degeneration
  • age-related macular degeneration
show more synonyms
Genetic modifications
Isogenic modification
4q25
p.Gly119Arg
Heterozygous