SCTCi014-A-1

IPS17-00056 Crispr clone O5

General

Cell Line

hPSCreg Name
SCTCi014-A-1
Alternative name(s)
IPS17-00056 Crispr clone O5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th November 2021
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Provider

Generator Radboudumc Stem Cell Technology Center (SCTC)
Owner Radboudumc - Department of Ophthalmology
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA11293804
CLO CLO_0103638

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Age-Related Macular Degeneration
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • ARMD
  • AMD
  • Age-Related Macular Degeneration
  • age-related macular degeneration
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
CNV analysis
no mutations found

External Databases (Donor)

BioSamples SAMEA10457739

Ethics

Also have a look at the ethics information for the parental line SCTCi014-A .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Synthego
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution not for commercial and clinical use

hIPSC Derivation

General

The source cell information can be found in the parental cell line SCTCi014-A.
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Selection criteria for clones The clones were picked manually with a capillary pipette and a stereomicroscope
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
HAND1
Yes
Desmin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
NCAM1
No
NESTIN
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 27

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Age-Related Macular Degeneration
Synonyms
  • ARMD
  • AMD
  • Age-Related Macular Degeneration
  • age-related macular degeneration
show more synonyms
Genetic modifications
CFI (target)
Isogenic modification
4q25
c.355G>A
p.Gly119Arg
Heterozygous