IPS17-00056 Crispr clone O5
SCTCi014-A-1
General
Cell Line |
|
hPSCreg name | SCTCi014-A-1 |
Cite as: | SCTCi014-A-1 (RRID:CVCL_C0FT) |
Alternative name(s) |
IPS17-00056 Crispr clone O5
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UNEWi026-A (SF116 clone 1) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration STBCi110-A (SFC116-03-01) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration UNEWi026-B (SF116 clone 2) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration UNEWi026-C (SF116 clone K) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration |
Last update | 30th November 2021 |
User feedback | |
Provider |
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Generator | Radboudumc Stem Cell Technology Center (SCTC) |
Owner | Radboudumc - Department of Ophthalmology |
Distributors | |
Derivation country | Netherlands |
External Databases |
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BioSamples | SAMEA11293804 |
Cellosaurus | CVCL_C0FT |
Wikidata | Q112930352 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
Yes
CNV analysis
no mutations found |
External Databases (Donor) |
|
BioSamples | SAMEA10457739 |
Ethics
Also have a look at the ethics information for the parental line
SCTCi014-A
.
Is there an MTA available for the cell line? | Yes |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Synthego |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
Constraints for use or distribution | not for commercial and clinical use |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SCTCi014-A.
|
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Passage number reprogrammed | 10 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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Selection criteria for clones | The clones were picked manually with a capillary pipette and a stereomicroscope |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™ Flex
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
27
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Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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