IPS18-00072

General#

Cell Line

hPSCreg Name SCTCi011-A
Alternative name(s)
IPS18-00072
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 3rd December 2020
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Provider

Generator Radboudumc Stem Cell Technology Center (SCTC)
Owner Radboudumc - Department of Ophthalmology
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA7807651
CLO CLO_0103128

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Additional restrictions:

Only in collaboration with the Radboudumc and after the signature of an MTA

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
age-related macular degeneration
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Macular Dystrophy
  • Degeneration, Macular
  • Age Related Maculopathies
  • MACULAR DEGENERATION NOS
  • Age related maculopathy
  • Age Related Macular Degeneration
  • Unspecified senile macular degeneration
  • Maculopathies, Age Related
  • MACULOPATHY AGE RELAT
  • AAMD - Age related macular degeneration
  • Maculopathies, Age-Related
  • Macular Dystrophies
  • ARMD
  • Amended
  • Dystrophies, Macular
  • Age-Related Maculopathy
  • Senile macular degeneration
  • SMD - Senile macular degeneration
  • Macular degeneration (senile) of retina, unspecified
  • Macular Degeneration, Age-Related
  • Degeneration, Age-Related Macular
  • Macular degeneration (disorder)
  • Macular Degenerations
  • Degenerations, Age-Related Macular
  • AGE RELAT MACULOPATHIES
  • Macular Degenerations, Age-Related
  • Maculopathy, Age-Related
  • AMD - Age-related macular degeneration
  • Maculopathy, Age Related
  • Dystrophy, Macular
  • Degenerations, Macular
  • Senile macular retinal degeneration
  • Age-related macular degeneration (disorder)
  • Age related macular degeneration (disorder) [Ambiguous]
  • AGE RELAT MACULOPATHY
  • MACULOPATHIES AGE RELAT
  • Age-Related Macular Degenerations
  • Age-Related Maculopathies
  • Senile macular degeneration of retina
  • ARMD - Age-related macular degeneration
  • Macular Degeneration
  • macular degeneration, age-related
  • AMD
  • age-related macular degeneration
  • age related maculopathy
  • age related Maculopathies
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH

External Databases (Donor)

BioSamples SAMEA7023725

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: The owner of the cell line can access the electronic health record
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? CMO Arnhem Nijmegen
Approval number 2007-158
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? CMO Arnhem Nijmegen
Approval number 2007-158
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Add Gene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution not for commercial and clinical use

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
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Source cell origin
Arm
The portion of the upper extremity between the shoulder and the elbow. For clinical purposes this term is also used to refer to the whole superior limb.
Synonyms
  • ARM
  • Arm
  • Brachium
  • Upper Arm
show more synonyms
Age of donor (at collection) 60-64
Collected in 2018

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones The clones were picked manually with a capillary pipette under a stereomicroscope
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
DNMT3B
Yes
SSEA-4
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
NCAM1
Yes
HAND1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
NCAM1
Yes
NESTIN
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 10

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)