HPSI0914i-fuhz_1

General#

Cell Line

hPSCreg Name WTSIi158-A
Alternative name(s)
HPSI0914i-fuhz_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA3313418
HipSci HPSI0914i-fuhz_1
Cellosaurus CVCL_AH56
ECACC 77650276
EBiSC WTSIi158-A

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Bardet-Biedl syndrome
Synonyms
  • BBS

External Databases (Donor)

BioSamples SAMEA2785488
HipSci HPSI-fuhz

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 30-34
Collected in 2015
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
26.232 1.474

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 12

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0914i-fuhz_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Imputed and phased genotypes
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Text file with probe intensities
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls