HPSI0714i-rajk_1

General

Cell Line

hPSCreg name WTSIi161-A
Cite as:
WTSIi161-A (RRID:CVCL_AG83)
Alternative name(s)
HPSI0714i-rajk_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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WTSIi147-A
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WTSIi151-A
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WTSIi152-A
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WTSIi158-A
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WTSIi165-A
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WTSIi414-B
(HPSI0614i-eisk_6)
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WTSIi405-B
(HPSI0614i-fovu_5)
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WTSIi458-B
(HPSI0614i-lipl_5)
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WTSIi402-B
(HPSI0714i-ouzk_4)
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WTSIi465-B
(HPSI0714i-xiry_1)
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WTSIi407-B
(HPSI0914i-hidy_3)
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WTSIi452-B
(HPSI0115i-xaka_1)
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WTSIi033-A
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Bardet-Biedl syndrome
WTSIi153-A
(HPSI0414i-puvg_1)
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Bardet-Biedl syndrome
WTSIi156-A
(HPSI0414i-xugr_2)
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Bardet-Biedl syndrome
WTSIi157-A
(HPSI0614i-eavo_1)
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Bardet-Biedl syndrome
WTSIi162-A
(HPSI0914i-lopq_1)
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WTSIi166-A
(HPSI0414i-wize_1)
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WTSIi034-A
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WTSIi035-A
(HPSI0913i-gooj_1)
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WTSIi149-A
(HPSI0913i-laia_4)
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Bardet-Biedl syndrome
WTSIi150-A
(HPSI0414i-jetz_1)
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Bardet-Biedl syndrome
WTSIi155-A
(HPSI0414i-hutq_1)
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Bardet-Biedl syndrome
WTSIi160-A
(HPSI0914i-verf_1)
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WTSIi163-A
(HPSI1014i-suok_3)
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WTSIi485-B
(HPSI0614i-kecw_4)
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WTSIi399-B
(HPSI0614i-gawh_12)
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WTSIi415-B
(HPSI0614i-laig_2)
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WTSIi457-B
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WTSIi420-B
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WTSIi036-A
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BCHNi001-A
(BCHi001-A)
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WTSIi576-A
(HPSI0216i-feht_1)
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WTSIi450-B
(HPSI0216i-aiid_6)
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Usher syndrome
WTSIi516-B
(HPSI0216i-iogu_6)
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Usher syndrome
WTSIi614-A
(HPSI0616i-rily_3)
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Spastic paraplegia
UMGWi001-B
(Z1 K3v3)
Donor diseases:
Floating-Harbor syndrome
WTSIi527-B
(HPSI0216i-heub_6)
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Usher syndrome
WTSIi474-B
(HPSI0216i-dewh_4)
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ESi055-A
(AS FiPS 2-Ep6F-28)
Donor diseases:
Alport syndrome
ESi056-A
(AS FiPS 3-Ep6F-9)
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Alport syndrome
WTSIi611-A
(HPSI0816i-neow_5)
Donor diseases:
Alport syndrome
WTSIi623-A
(HPSI0516i-yist_3)
Donor diseases:
Alport syndrome
WTSIi620-A
(HPSI0616i-uegn_3)
Donor diseases:
Spastic paraplegia
WTSIi624-A
(HPSI0616i-uofs_5)
Donor diseases:
Spastic paraplegia
Last update 9th August 2019
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA3355533
HipSci HPSI0714i-rajk_1
Cellosaurus CVCL_AG83
EBiSC WTSIi161-A
Wikidata Q54891329

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 55-59
Ethnicity White British

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2658103
HipSci HPSI-rajk

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: HipSci (http://www.hipsci.org/)
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink HipSci (http://www.hipsci.org/)
Is there other documentation provided to the donor for consenting purposes? No
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? NRES Committee Yorkshire & The Humber - Leeds West
Approval number 08/H0713/82
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59
Collected in 2015
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score
18.247 1.698

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 32

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-rajk_1
Number of regions different from primary tissue: 3; Length of differences from primary tissue: 2
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Text file with probe intensities
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts