HPSI0516i-pews_2

General

Cell Line

hPSCreg name WTSIi522-B
Cite as:
WTSIi522-B (RRID:CVCL_IY07)
Alternative name(s)
HPSI0516i-pews_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi522-A
(HPSI0516i-pews_3)
Donor diseases:
Usher syndrome
WTSIi576-A
(HPSI0216i-feht_1)
Donor diseases:
Usher syndrome
WTSIi450-B
(HPSI0216i-aiid_6)
Donor diseases:
Usher syndrome
WTSIi516-B
(HPSI0216i-iogu_6)
Donor diseases:
Usher syndrome
WTSIi447-B
(HPSI0416i-zige_2)
Donor diseases:
Usher syndrome
WTSIi454-B
(HPSI0516i-iool_4)
Donor diseases:
Usher syndrome
WTSIi447-A
(HPSI0416i-zige_5)
Donor diseases:
Usher syndrome
WTSIi450-A
(HPSI0216i-aiid_1)
Donor diseases:
Usher syndrome
WTSIi454-A
(HPSI0516i-iool_6)
Donor diseases:
Usher syndrome
WTSIi487-A
(HPSI0516i-goek_4)
Donor diseases:
Usher syndrome
WTSIi516-A
(HPSI0216i-iogu_5)
Donor diseases:
Usher syndrome
WTSIi527-B
(HPSI0216i-heub_6)
Donor diseases:
Usher syndrome
WTSIi474-B
(HPSI0216i-dewh_4)
Donor diseases:
Usher syndrome
WTSIi510-B
(HPSI0216i-xanu_6)
Donor diseases:
Usher syndrome
WTSIi548-B
(HPSI0416i-mefc_1)
Donor diseases:
Usher syndrome
WTSIi488-B
(HPSI0516i-oazw_3)
Donor diseases:
Usher syndrome
WTSIi464-B
(HPSI0516i-eitu_4)
Donor diseases:
Usher syndrome
WTSIi486-B
(HPSI0516i-hapz_2)
Donor diseases:
Usher syndrome
WTSIi669-A
(HPSI0816i-aorf_1)
Donor diseases:
Usher syndrome
WTSIi669-B
(HPSI0816i-aorf_2)
Donor diseases:
Usher syndrome
WTSIi453-A
(HPSI0416i-fiwt_3)
Donor diseases:
Usher syndrome
WTSIi464-A
(HPSI0516i-eitu_3)
Donor diseases:
Usher syndrome
WTSIi474-A
(HPSI0216i-dewh_6)
Donor diseases:
Usher syndrome
WTSIi486-A
(HPSI0516i-hapz_3)
Donor diseases:
Usher syndrome
WTSIi488-A
(HPSI0516i-oazw_4)
Donor diseases:
Usher syndrome
WTSIi510-A
(HPSI0216i-xanu_5)
Donor diseases:
Usher syndrome
WTSIi527-A
(HPSI0216i-heub_1)
Donor diseases:
Usher syndrome
WTSIi548-A
(HPSI0416i-mefc_2)
Donor diseases:
Usher syndrome
RIi011-A
(USH.1.H.iPSC.6)
Donor diseases:
Usher syndrome
WTSIi152-A
(HPSI0414i-eafb_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi414-B
(HPSI0614i-eisk_6)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-B
(HPSI0614i-fovu_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi458-B
(HPSI0614i-lipl_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi573-A
(HPSI0115i-qaqp_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi402-B
(HPSI0714i-ouzk_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi465-B
(HPSI0714i-xiry_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi407-B
(HPSI0914i-hidy_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi452-B
(HPSI0115i-xaka_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi573-B
(HPSI0115i-qaqp_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi402-A
(HPSI0714i-ouzk_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi403-A
(HPSI0913i-rufg_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-A
(HPSI0614i-fovu_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi406-A
(HPSI0614i-mesr_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi407-A
(HPSI0914i-hidy_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi414-A
(HPSI0614i-eisk_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi147-A
(HPSI1013i-mijn_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi148-A
(HPSI0114i-veui_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi151-A
(HPSI0414i-fixm_2)
Donor diseases:
Bardet-Biedl syndrome
Last update 6th October 2021
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA4563382
HipSci HPSI0516i-pews_2
Cellosaurus CVCL_IY07
Wikidata Q54890976

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 40-44
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Retinitis pigmentosa-deafness syndrome
  • USH
  • Retinitis pigmentosa-hearing loss syndrome

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4453764

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Is there other documentation provided to the donor for consenting purposes? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Hampstead Research Ethics Committee
Approval number 13/LO/0171
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 40-44
Collected in 2016
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
22.158 1.433

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0516i-pews_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 4
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003528
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003528
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003543
Splice-aware STAR alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003543
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003528
BWA alignment