BNH1 _iPSC_WT_8
UMGWi004-A
General
Cell Line |
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| hPSCreg name | UMGWi004-A |
| Cite as: | UMGWi004-A |
| Alternative name(s) |
BNH1 _iPSC_WT_8
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UMGWi004-B (BNH1 _iPSC_WT_48) |
| Last update | 23rd February 2024 |
| User feedback | |
Provider |
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| Generator | Department of Functional Genomics - Human Molecular Genetics (UMGW) |
External Databases |
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| BioSamples | SAMEA115116691 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has son:
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External Databases (Donor) |
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| BioSamples | SAMEA115116692 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | SensID GmbH, Schillingallee 68, 18057 Rostock |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | SensID GmbH, Schillingallee 68, 18057 Rostock |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics Committee of the University of Greifswald |
| Approval number | BB047/14 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Ethics Committee of the University of Greifswald |
| Approval number | BB047/14 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Synonyms
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| Source cell origin | |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | PCR with primers specific for OriP and EBNA sequences present in all three vectors used for reprogramming (Addgene 27077, 27078, 27080) |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
Morphology
SOX17 expression in embryoid bodies relative to iPSC
AFP Immunofluorescence - merge with DAPI
In vitro spontaneous differentiation
Morphology
TBXT (T) expression in embryoid bodies relative to iPSC
SMA Immunofluorescence - merge with DAPI
In vitro spontaneous differentiation
Morphology
PAX6 expression in embryoid body relative to iPSC
TUBB3 Immunofluorescence - merge with DAPI
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46 XY
Karyotyping method:
low-coverage whole genome sequencing (lcWGS)
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Other Genotyping (Cell Line) |
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