L_mut_FLAG Clone 15
DHMi005-A-7
General
iPSC Line |
|
| hPSCreg name | DHMi005-A-7 |
| Cite as: | DHMi005-A-7 (RRID:CVCL_D0E2) |
| Alternative name(s) |
L_mut_FLAG Clone 15
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
DHMi005-A-5 (L_mut_FLAG Clone 4) DHMi005-A-6 (L_mut_FLAG Clone 14) DHMi005-A (Control_L) DHMi005-A-1 (L_mut) DHMi005-A-2 (L_FLAG Clone 5) DHMi005-A-3 (L_FLAG Clone 6) DHMi005-A-4 (L_FLAG Clone 7) DHMi005-A-8 (L Delta B2M Clone 6) DHMi005-A-9 (L Delta B2M Clone 11) UCSFi001-A-1M (iN-SRCAP-R2444-3) UCSFi001-A-1N (iN-SRCAP-R2444-5) UMGWi004-B-1 (BNH1_iPSC_FHS1_cr_well7_JR) UMGWi004-B-2 (BNH1_iPSC_FHS1_cr_well12_JR) ITXi006-A-1 (IM-R406W) BIONi010-C-10 (HNF1AP291fsinsC +/- 54-5) BIONi010-C-11 (HNF1AP291fsinsC -/- 66-1) BIONi010-C-12 (HNF4ApR309C -/- 2-4) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) CBIGi001-A-29 (ATAXIN3-KO/AIW002-02, IPSC0101) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) BIONi010-C-65 (BiONI010-C-O16) ICANi002-A-5 (ICAN-PKP2-H695-W11) BIONi010-C-66 (BIONi010-C-N7) |
| Last update | 20th October 2022 |
| User feedback | |
Provider |
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| Generator | German Heart Center Munich (DHM) |
| Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
| Distributors | |
| Derivation country | Germany |
External iPSC Databases |
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| BioSamples | SAMEA111493368 |
| Cellosaurus | CVCL_D0E2 |
| Wikidata | Q123031168 |
General iPSC Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | No abnormalities have been described with regard to the family history. |
| Is the medical history available upon request? | no, healthy proband |
| Is clinical information available? | no, healthy proband |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA12738677 |
Ethics
Also have a look at the ethics information for the parental line
DHMi005-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Genscript, Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
DHMi005-A.
|
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
ReLeSR
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
Morphology pictures
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
46, XY
Passage number: 56
Karyotyping method:
G-Banding
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Other Genotyping (iPSC Line) |
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