DHMi005-A-7

L_mut_FLAG Clone 15

General

Cell Line

hPSCreg Name
DHMi005-A-7
Alternative name(s)
L_mut_FLAG Clone 15
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 20th October 2022
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Provider

Generator German Heart Center Munich (DHM)
Owner German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA111493368
CLO CLO_0104124

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history No abnormalities have been described with regard to the family history.
Is the medical history available upon request? no, healthy proband
Is clinical information available? no, healthy proband

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12738677

Ethics

Also have a look at the ethics information for the parental line DHMi005-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Genscript, Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line DHMi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT4
Yes
KLF4
Yes
SOX2
Yes
c-MYC
Yes
NANOG
Yes
REX1
Yes
TRA 1-81
Yes
SOX2
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 56
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Holt-Oram Syndrome
Isogenic modification, Chromosome location: TBX5 gene, Chromosome 12, NC_000012.12, Mutation c920_C>A was inserted in the healthy motherline DHMi005-A, Proteinsequence change: NP_852259:p.Pro85Thr, heterozygous ( DOI: 10.1016/j.scr.2022.102691) Additonally, a Flag tag insertion in the last Exon of TBX5 in front of the stop codon
Synonyms
  • Holt-Oram Syndrome
Genetic modifications
TBX5 (target)
Variant
12q24
NM_000192.3:c.52908_52909insGACTACAAGGACGACGATGACAAA
NP_852259:p.S518_ins.DYKDDDDK
Heterozygous
Insertion of an Flag-Tag in the last Exon of TBX5 in front of the stop codon of a CRISPR/Cas9 edited iPS line (DOI: 10.1016/j.scr.2022.102691)