L_FLAG Clone 7
DHMi005-A-4
General
Cell Line |
|
| hPSCreg name | DHMi005-A-4 |
| Cite as: | DHMi005-A-4 (RRID:CVCL_C0IT) |
| Alternative name(s) |
L_FLAG Clone 7
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
DHMi005-A-2 (L_FLAG Clone 5) DHMi005-A-3 (L_FLAG Clone 6) DHMi005-A (Control_L) DHMi005-A-8 (L Delta B2M Clone 6) DHMi005-A-9 (L Delta B2M Clone 11) DHMi005-A-5 (L_mut_FLAG Clone 4) DHMi005-A-1 (L_mut) DHMi005-A-6 (L_mut_FLAG Clone 14) DHMi005-A-7 (L_mut_FLAG Clone 15) |
| Last update | 23rd May 2023 |
| User feedback | |
Provider |
|
| Generator |
German Heart Center Munich (DHM)
Contact:
German Heart Center Munich (DHM) |
| Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA14493160 |
| Cellosaurus | CVCL_C0IT |
| Wikidata | Q112929456 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | No abnormalities have been described with regard to the family history. |
| Is the medical history available upon request? | no, healthy proband |
| Is clinical information available? | no, healthy proband |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA12738677 |
Ethics
Also have a look at the ethics information for the parental line
DHMi005-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Genscript, Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
DHMi005-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
ReLeSR
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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