HOS_1460corr 127 Clone 31
DHMi004-A-1
General
Cell Line |
|
| hPSCreg name | DHMi004-A-1 |
| Cite as: | DHMi004-A-1 (RRID:CVCL_D0DX) |
| Alternative name(s) |
HOS_1460corr 127 Clone 31
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 30th May 2023 |
| User feedback | |
Provider |
|
| Generator | German Heart Center Munich (DHM) |
| Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA112137054 |
| Cellosaurus | CVCL_D0DX |
| Wikidata | Q123031160 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Family history | healthy parents, female sibling with the diagnose of atrial septal defect II |
| Is the medical history available upon request? | yes |
| Is clinical information available? | Clinical information is available upon request. |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA12616340 |
Ethics
Also have a look at the ethics information for the parental line
DHMi004-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Integrated DNA Technologies |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
DHMi004-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | Sendai Footprinting |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | individual colonies were manually picked, expanded and sequenced for correction of TBX5 mutation |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
ReLeSR
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
|
Login to share your feedback, experiences or results with the research community.