DHMi004-A-2

HOS_1460_FLAG Clone 4

General

Cell Line

hPSCreg Name
DHMi004-A-2
Alternative name(s)
HOS_1460_FLAG Clone 4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd November 2022
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Provider

Generator German Heart Center Munich (DHM)
Owner German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA112152464

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Holt-Oram Syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Holt-Oram Syndrome
Genetic variants
TBX5 gene, Chromosome 12, NC_000012.12 (114353911..114408708, complete)
NM_000192.3: c.920_C>A
NP_000183.2:p.Pro85Thr
Heterozygous
PMID: 27652283
All coding exons of the TBX5 gene were directly sequenced from genomic DNA of the patient. In exon 4 the single-nucleotide change was detected.
Family history healthy parents, female sibling with the diagnose of atrial septal defect II
Is the medical history available upon request? yes
Is clinical information available? Clinical information is available upon request.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA12616340

Ethics

Also have a look at the ethics information for the parental line DHMi004-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Thermofisher
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line DHMi004-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection Sendai footprinting

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line DHMi004-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Variant
12q24
NM_000192.3:c.52908_52909insGACTACAAGGACGACGATGACAAA
NP_852259:p.S518_ins.DYKDDDDK
Heterozygous
Insertion of an Flag-Taq in the last Exon of TBX5 in front of the stop codon