HOS_1460_FLAG Clone 4
DHMi004-A-2
General
Cell Line |
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| hPSCreg name | DHMi004-A-2 |
| Cite as: | DHMi004-A-2 (RRID:CVCL_D0DY) |
| Alternative name(s) |
HOS_1460_FLAG Clone 4
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 22nd November 2022 |
| User feedback | |
Provider |
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| Generator | German Heart Center Munich (DHM) |
| Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA112152464 |
| Cellosaurus | CVCL_D0DY |
| Wikidata | Q123031161 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | healthy parents, female sibling with the diagnose of atrial septal defect II |
| Is the medical history available upon request? | yes |
| Is clinical information available? | Clinical information is available upon request. |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA12616340 |
Ethics
Also have a look at the ethics information for the parental line
DHMi004-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Thermofisher |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
DHMi004-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | Sendai footprinting |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
ReLeSR
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line DHMi004-A .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Genetic modifications not related to a disease |
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