Generation of two CRISPR/Cas edited human induced pluripotent stem cell lines (DHMi005-A-3 and DHMi005-A-4) carrying a FLAG-tag after exon 9 of the TBX5 gene
Summary
Although TBX5 plays a major role during human cardiogenesis and initiates and controls limb development, many of its interactions with genomic DNA and the resulting biological consequences are not well known. Existing anti-TBX5-antibodies work very inefficiently in certain applications such as ChIP-Seq analysis. To circumvent this drawback, we introduced a FLAG-tag sequence into the TBX5 locus at the end of exon 9 prior to the stop codon by CRISPR/Cas9. The expressed TBX5-FLAG fusion protein can effectively be precipitated by anti-FLAG antibodies. Therefore, these gene-edited iPSC lines represent powerful cellular in vitro tools to unravel TBX5:DNA interactions in detail. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Lahm H, Stieglbauer S, Neb I, Doppler SA, Schneider S, Dzilic E, Lange R, Krane M, Dreßen M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Feb;66:103011 |
| PubMed | 36610218 |
| DOI | 10.1016/j.scr.2022.103011 |