DHMi005-A-1

L_mut

General#

Cell Line

hPSCreg Name
DHMi005-A-1
Alternative name(s)
L_mut
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 15th December 2021
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Provider

Generator German Heart Center Munich (DHM)
Owner German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA11744856

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Holt-Oram Syndrome
Synonyms
  • Holt-Oram Syndrome
Family history No abnormalities have been described with regard to the family history.
Is the medical history available upon request? no, healthy proband
Is clinical information available? no, healthy proband

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA11305579

Ethics#

Also have a look at the ethics information for the parental line DHMi005-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

The source cell information can be found in the parental cell line DHMi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line DHMi005-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 23
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Holt-Oram Syndrome
Synonyms
  • Holt-Oram Syndrome
Genetic modifications
Isogenic modification
TBX5 gene, Chromosome 12, NC_000012.12 (114353911..114408708, complete)
NM_0000192.3: c920_C>A
NP_852259:p.Pro85Thr
Heterozygous
Mutated