DHMi005-A-1

L_mut

General

Cell Line

hPSCreg name DHMi005-A-1
Cite as:
DHMi005-A-1 (RRID:CVCL_B5SK)
Alternative name(s)
L_mut
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
DHMi005-A
(Control_L)
DHMi005-A-5
(L_mut_FLAG Clone 4)
DHMi005-A-6
(L_mut_FLAG Clone 14)
DHMi005-A-7
(L_mut_FLAG Clone 15)
DHMi005-A-2
(L_FLAG Clone 5)
DHMi005-A-3
(L_FLAG Clone 6)
DHMi005-A-4
(L_FLAG Clone 7)
DHMi005-A-8
(L Delta B2M Clone 6)
DHMi005-A-9
(L Delta B2M Clone 11)
ITXi006-A-1
(IM-R406W)
UCSFi001-A-49
(YH653-MUT-1C8-HET, DYT1-HET-1C8)
UCSFi001-A-50
(YH653-MUT-H6-HET, DYT1-HET-H6)
UCSFi001-A-51
(YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2)
BIONi010-C-10
(HNF1AP291fsinsC +/- 54-5)
BIONi010-C-11
(HNF1AP291fsinsC -/- 66-1)
BIONi010-C-12
(HNF4ApR309C -/- 2-4)
BIONi010-C-65
(BiONI010-C-O16)
ICANi002-A-5
(ICAN-PKP2-H695-W11)
BIONi010-C-66
(BIONi010-C-N7)
Last update 28th January 2022
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Provider

Generator German Heart Center Munich (DHM)
Owner German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA11744856
Cellosaurus CVCL_B5SK
Wikidata Q111733156

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history No abnormalities have been described with regard to the family history.
Is the medical history available upon request? no, healthy proband
Is clinical information available? no, healthy proband

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12738677

Ethics

Also have a look at the ethics information for the parental line DHMi005-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line DHMi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line DHMi005-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 23
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Holt-Oram Syndrome
Genetic modifications
Isogenic modification
TBX5 gene, Chromosome 12, NC_000012.12 (114353911..114408708, complete)
NM_0000192.3: c920_C>A
NP_852259:p.Pro85Thr
Heterozygous
Mutated