ICANi002-A-5

ICAN-PKP2-H695-W11

General

Cell Line

hPSCreg name ICANi002-A-5
Cite as:
ICANi002-A-5 (RRID:CVCL_D6MB)
Alternative name(s)
ICAN-PKP2-H695-W11
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
ICANi002-A
(ICAN-403.3)
LUMCi027-A-1
(iso01LUMC0153iPKP03, LUMC0153iPKP03corr#22)
Donor's gene variants:
PKP2, PKP2
Donor diseases:
arrhythmogenic right ventricular dysplasia 9
ICANi002-A-3
(ICAN-BAG3-V468MC34)
ICANi002-A-4
(ICAN_BAG3_M468MC19)
ICANi002-A-1
(ICAN-FLNC42.1)
ICANi002-A-2
(SCN5A-Clone 5)
ITXi006-A-1
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UCSFi001-A-49
(YH653-MUT-1C8-HET, DYT1-HET-1C8)
UCSFi001-A-50
(YH653-MUT-H6-HET, DYT1-HET-H6)
UCSFi001-A-51
(YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2)
UCSFi001-A-64
(P633L RBM20 iPSCs)
CMUi002-A-1
(NONO-KO-iPSCs)
DHMi005-A-5
(L_mut_FLAG Clone 4)
DHMi005-A-6
(L_mut_FLAG Clone 14)
DHMi005-A-7
(L_mut_FLAG Clone 15)
BIONi010-C-10
(HNF1AP291fsinsC +/- 54-5)
BIONi010-C-11
(HNF1AP291fsinsC -/- 66-1)
BIONi010-C-12
(HNF4ApR309C -/- 2-4)
Last update 11th March 2024
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Provider

Generator INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Owner INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Distributors
Derivation country France

External Databases

BioSamples SAMEA114772233
Cellosaurus CVCL_D6MB
Wikidata Q127382311

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12575407

Ethics

Also have a look at the ethics information for the parental line ICANi002-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line ICANi002-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Alkaline Phosphatase
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
TNNT2
Yes
ACTN2
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotype-ICAN-PKP2-H695-W11
Passage number: 47
Karyotyping method: mFISH

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Arrhythmogenic right ventricular cardiomyopathy 9
Synonyms
  • ARVC9
  • ARVD9
  • PKP2 familial isolated arrhythmogenic right ventricular dysplasia
  • arrhythmogenic right ventricular cardiomyopathy 9
  • arrhythmogenic right ventricular dysplasia 9
  • arrhythmogenic right ventricular dysplasia type 9
  • arrhythmogenic right ventricular dysplasia, familial, type 9
  • familial arrhythmogenic right ventricular dysplasia 9
  • familial isolated arrhythmogenic right ventricular dysplasia caused by mutation in PKP2
  • arrhythmogenic right ventricular dysplasia, familial, 9
show more synonyms
Genetic modifications
PKP2 (target)
Isogenic modification
12p11.21
NM_001005242.3:c.2083_2089delinsCATGTTG
NP_001005242.2:p.His695ValfsTer4
Heterozygous
PKP2 frame-shifts and non-sense mutations are the most frequent genetic cause of ARVC (50%). The deletion carried by the described clone is a frame shift leading to ~50% decreased expression of the protein in iPS-cardiomyocytes cells.
Mutated