ICAN-PKP2-H695-W11
ICANi002-A-5
General
Cell Line |
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| hPSCreg name | ICANi002-A-5 |
| Cite as: | ICANi002-A-5 (RRID:CVCL_D6MB) |
| Alternative name(s) |
ICAN-PKP2-H695-W11
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
ICANi002-A (ICAN-403.3) LUMCi027-A-1 (iso01LUMC0153iPKP03, LUMC0153iPKP03corr#22) Donor's gene variants: PKP2, PKP2 Donor diseases: arrhythmogenic right ventricular dysplasia 9 ICANi002-A-3 (ICAN-BAG3-V468MC34) ICANi002-A-4 (ICAN_BAG3_M468MC19) ICANi002-A-1 (ICAN-FLNC42.1) ICANi002-A-2 (SCN5A-Clone 5) ITXi006-A-1 (IM-R406W) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) UCSFi001-A-64 (P633L RBM20 iPSCs) CMUi002-A-1 (NONO-KO-iPSCs) DHMi005-A-5 (L_mut_FLAG Clone 4) DHMi005-A-1 (L_mut) DHMi005-A-6 (L_mut_FLAG Clone 14) DHMi005-A-7 (L_mut_FLAG Clone 15) BIONi010-C-10 (HNF1AP291fsinsC +/- 54-5) BIONi010-C-11 (HNF1AP291fsinsC -/- 66-1) BIONi010-C-12 (HNF4ApR309C -/- 2-4) |
| Last update | 11th March 2024 |
| User feedback | |
Provider |
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| Generator | INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN) |
| Owner | INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN) |
| Distributors | |
| Derivation country | France |
External Databases |
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| BioSamples | SAMEA114772233 |
| Cellosaurus | CVCL_D6MB |
| Wikidata | Q127382311 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA12575407 |
Ethics
Also have a look at the ethics information for the parental line
ICANi002-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
ICANi002-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| Alkaline Phosphatase |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Scorecard
Differentiation Potency
In vitro directed differentiation
Morphology
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Karyotype-ICAN-PKP2-H695-W11
Passage number: 47
Karyotyping method:
mFISH
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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