ICANi002-A-1

ICAN-FLNC42.1

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name
ICANi002-A-1
Alternative name(s)
ICAN-FLNC42.1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 1st October 2021
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Provider

Generator INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Owner INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Distributors
Derivation country France

External Databases

BioSamples SAMEA10203007
CLO CLO_0103545

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA10203006

Ethics#

Also have a look at the ethics information for the parental line ICANi002-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line ICANi002-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotpye ICAN-FLNC 42.1 P39
Passage number: 41
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
FLNC (target)
Variant
7q32.1
NM_001458.4:c.7121_7135+3del
NP_001120959.1:p.(Val2333_Pro2378del)
Homozygous
Exon skipping mutation induced by NHEJ after CRISPR/Cas9 directed edition. Mutation type very probably associated with Dilated Cardiomyopathy phenotype (http://www.ebi.ac.uk/efo/EFO_0000407)