ICANi002-A-2

SCN5A-Clone 5

General

Cell Line

hPSCreg Name
ICANi002-A-2
Alternative name(s)
SCN5A-Clone 5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th April 2022
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Provider

Generator INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Owner INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Distributors
Derivation country France

External Databases

BioSamples SAMEA11293803
Cellosaurus CVCL_B5EZ
CLO CLO_0103880

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

Material Transfer Agreement (MTA) and collaboration with Dr Nathalie Neyroud (UMRS-1166).

Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12575407

Ethics

Also have a look at the ethics information for the parental line ICANi002-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line ICANi002-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-4
Yes
Alkaline Phosphatase
Yes
Score:
Marker Present Absent
mCpG
OCT4
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 31
Karyotyping method: mFISH

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
SCN5A (target)
Variant
3p22.2
NM_198056.2(SCN5A):c.45del
NP_932173.1_p.(Arg15Serfs*82)
Heterozygous
Heterozygous frameshift mutation in SCN5A induced by NHEJ after CRISPR/Cas9 direct edition