SCN5A-Clone 5
ICANi002-A-2
General
Cell Line |
|
| hPSCreg name | ICANi002-A-2 |
| Cite as: | ICANi002-A-2 (RRID:CVCL_B5EZ) |
| Alternative name(s) |
SCN5A-Clone 5
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
ICANi002-A (ICAN-403.3) ICANi002-A-3 (ICAN-BAG3-V468MC34) ICANi002-A-4 (ICAN_BAG3_M468MC19) ICANi002-A-1 (ICAN-FLNC42.1) ICANi002-A-5 (ICAN-PKP2-H695-W11) |
| Last update | 5th April 2022 |
| User feedback | |
Provider |
|
| Generator | INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN) |
| Owner | INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN) |
| Distributors | |
| Derivation country | France |
External Databases |
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| BioSamples | SAMEA11293803 |
| Cellosaurus | CVCL_B5EZ |
| Wikidata | Q110432903 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
Material Transfer Agreement (MTA) and collaboration with Dr Nathalie Neyroud (UMRS-1166). |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA12575407 |
Ethics
Also have a look at the ethics information for the parental line
ICANi002-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
ICANi002-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-4 |
Yes |
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| Alkaline Phosphatase |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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