ICAN_BAG3_M468MC19
ICANi002-A-4
General
Cell Line |
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| hPSCreg name | ICANi002-A-4 |
| Cite as: | ICANi002-A-4 |
| Alternative name(s) |
ICAN_BAG3_M468MC19
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
ICANi002-A-3 (ICAN-BAG3-V468MC34) ICANi002-A (ICAN-403.3) ICANi002-A-1 (ICAN-FLNC42.1) ICANi002-A-5 (ICAN-PKP2-H695-W11) ICANi002-A-2 (SCN5A-Clone 5) |
| Last update | 16th January 2024 |
| User feedback | |
Provider |
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| Generator | INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN) |
| Owner | INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN) |
| Distributors | |
| Derivation country | France |
External Databases |
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| BioSamples | SAMEA114332063 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA12575407 |
Ethics
Also have a look at the ethics information for the parental line
ICANi002-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
ICANi002-A.
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| Passage number reprogrammed | 24 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Morphology pictures
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Differentiation Potency
In vitro directed differentiation
Morphology
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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