ICANi002-A-6

ICAN-ENTREP1-KO-5.3.14

General

Cell Line

hPSCreg name ICANi002-A-6
Cite as:
ICANi002-A-6
Alternative name(s)
ICAN-ENTREP1-KO-5.3.14
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 31st July 2025
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Provider

Generator INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Owner INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Distributors
Derivation country France

External Databases

BioSamples SAMEA118573735

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12575407

Ethics

Also have a look at the ethics information for the parental line ICANi002-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line ICANi002-A.
Passage number reprogrammed 24

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Self-renewal
Unknown
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Differentiation Potency
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
ACTN2
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 42
Karyotyping method: RHG

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
ENTREP1 (target)
Gene knock-out
9q21.12
FAM189A2/ENTREP1 is a transmembrane protein, ubiquitously expressed. It is downregulated in various cancers and its reduced expression is linked to poor prognosis and disease progression.Through genome wide association studies and Burden test, variants have been implicated in cardiovascular diseases. An ENTREP1-deficient cell line was developed to investigate its roles in human cells.
CRISPR-associated (CRISPR/Cas) System