WAe009-A-28
General#
Cell Line |
|
| hPSCreg Name | WAe009-A-28 |
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 15th June 2020 |
| User feedback | |
Provider |
|
| Generator | Institute For Stem Cell Biology and Regenerative Medicine (INSTEM) |
External Databases |
|
| BioSamples | SAMEA6703338 |
| Cellosaurus | CVCL_ZB42 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
|
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7768918 |
Ethics#
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | Yes |
hESC Derivation#
| Date of derivation | 2017-05-12 |
| Supernumerary embryos from IVF treatment? |
No
Other hESC source:
Purchased from WiCell
|
| PGD Embryo? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
|
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
RT-PCRs and Immunostainings for Oct4, Sox2 and Nanog.
Differentiation Potency
In vitro spontaneous differentiation
Protocol or reference
Mesoderm Differentiation.jpg
Alpha Smooth Muscle Actin Staining
In vitro directed differentiation
Protocol or reference
Neural Differentiation.jpg
Differentiation into Nestin positive NSCs, Neurons and glia
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
|
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