WAe009-A-99

dCas9-p300 H9 21

General

Cell Line

hPSCreg Name
WAe009-A-99
Alternative name(s)
dCas9-p300 H9 21
Cell line type Human embryonic stem cell (hESC)
Last update 18th September 2022
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Provider

Generator Korea Institute of Toxicology (KIT)

External Databases

BioSamples SAMEA111365973
CLO CLO_0104044

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
DESMIN
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
NESTIN
Yes
TUBB
Unknown

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 53

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
(unknown disease)
Genetic modifications
Transgene expression
Lentiviral plasmid: pLV-dCas9-p300-P2A-PuroR, Addgene #83889
Viral
Lentivirus
Genetic modifications not related to a disease
Transgene expression
Lentiviral plasmid: pLV-dCas9-p300-P2A-PuroR, Addgene #83889
Viral
Lentivirus