Establishment of a human embryonic stem cell line, WAe009-A-99, with constitutive expression of the dCas9-p300 fusion protein


CRISPR/Cas9-based transcriptional regulation systems can induce the site-specific activation or repression of endogenous genes. p300 is a transcriptional co-activator that functions as a histone acetyltransferase that regulates gene transcription via chromatin remodeling. Here, we generated a human embryonic stem cell line stably expressing catalytically dead Cas9 (dCas9) fused to the catalytic core domain of human p300 via lentiviral transduction. This cell line can be used for locus-specific histone acetylation in combination with guide RNAs, and is a valuable tool for gene regulation in stem cell research. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Kang EH, Kim JW, Noh H, Park SH, Park HJ, Yoon S, Kim H
Journal Stem cell research
Publication Date 2022 Nov 30;66:102986
PubMed 36493708
DOI 10.1016/j.scr.2022.102986

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