Generation of a homozygous COX6A2 knockout human embryonic stem cell line (WAe009-A-47) via an epiCRISPR/Cas9 system


COX6A2 protein is a structural subunit of Complex IV (CIV/Cytochrome c oxidase/COX) in the mitochondrial respiratory chain. It is mainly expressed in the heart and skeletal muscle, also in some interneurons, regulating the assembly and catalytic activity of CIV. Its mutations can lead to COX deficiency, causing human myopathies, and maybe a potential cause of neurological abnormalities. Here, we used the CRISPR/Cas9 editing system to establish a homozygous COX6A2 knockout (COX6A2-KO) human embryonic stem cell (hESC) line. This COX6A2-KO hESC has normal morphology, pluripotency, and karyotype, which can differentiate into three germ layers in vivo. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Hang C, Song Y, Wu F, Dong T, Jiang M, Saleem A, Zhang S, Chang Y, Lu W, Cui M
Journal Stem cell research
Publication Date 2021 Jan 4;50:102152
PubMed 33418201
DOI 10.1016/j.scr.2020.102152

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