PDK1 KO
The cell line is not validated yet.
WAe009-A-2K
General
Cell Line |
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hPSCreg name | WAe009-A-2K |
Cite as: | WAe009-A-2K |
Alternative name(s) |
PDK1 KO
|
Cell line type | Human embryonic stem cell (hESC) |
Similar lines | No similar lines found. |
Last update | 10th October 2024 |
User feedback | |
Provider |
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Generator | Anzhen Hospital Capital Medical university (ANZH) |
Owner | Anzhen Hospital Capital Medical university (ANZH) |
Distributors | |
Derivation country | China |
External Databases |
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BioSamples | SAMEA115991981 |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
|
Family history | NO |
Is the medical history available upon request? | NO |
Is clinical information available? | NO |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
Is there an MTA available for the cell line? | No |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
Surface coating | Matrigel/Geltrex |
Passage method | Enzyme-free cell dissociation |
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
SSEA-4 |
Yes |
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NANOG |
Yes |
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TRA 1-60 |
Yes |
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Marker | Present | Absent |
mCpG | ||
OCT4 | X |
Report
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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