WAe009-A-2K

PDK1 KO

The cell line is not validated yet.

General

Cell Line

hPSCreg name WAe009-A-2K
Cite as:
WAe009-A-2K
Alternative name(s)
PDK1 KO
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 10th October 2024
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Provider

Generator Anzhen Hospital Capital Medical university (ANZH)
Owner Anzhen Hospital Capital Medical university (ANZH)
Distributors
Derivation country China

External Databases

BioSamples SAMEA115991981

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
O2 Concentration 21 %
CO2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
NANOG
Yes
TRA 1-60
Yes
Marker Present Absent
mCpG
OCT4 X
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Kidney
Ont Id: NCIT_C12415
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Protocol or reference
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vivo teratoma
Protocol or reference

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • diabetes
Genetic modifications
Gene knock-out
2q31.1
CRISPR-associated (CRISPR/Cas) System