Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system


The tissue inhibitors of metalloproteinases 3 (TIMP3) play an essential role in the tumorigenesis of human pancreatic endocrine tumors and Sorsby fundus dystrophy. To further investigate the significance of TIMP3 in disease, we used CRISPR/Cas9 to create a TIMP3 knock out human embryonic stem cell line (WAe009-A-89) that can differentiate into any desired cell type. Our results show that the WAe009-A-89 cell line retains the typical colony form and normal karyotype of stem cells. The cells strongly expressed pluripotency markers and could differentiate into tissues of all three germ layers in vivo. This cell line allowed exploring the role of the TIMP3 gene in related diseases. Copyright © 2023. Published by Elsevier B.V.

Authors Yi T, Liu J, Zhang S, He Y, Han J
Journal Stem cell research
Publication Date 2023 Mar;67:103034
PubMed 36716678
DOI 10.1016/j.scr.2023.103034

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