Generation of a heterozygous FUS-Q290X knock in human embryonic stem cell line (WAe009-A-83) using CRISPR/Cas9 system


Fused in Sarcoma (FUS) gene encodes FUS RNA binding protein, a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein complex, which is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm, and it has been implicated in regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. FUS gene mutations result in amyotrophic lateral sclerosis and Liposarcoma. This heterozygous FUS-Q290X knock in hESC line will be a valuable tool to investigate the disease mechanisms of amyotrophic lateral sclerosis and Liposarcoma. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang Y, Zhu J, Dai Y, Wang L, Liu R, Guo X
Journal Stem cell research
Publication Date 2022 Apr;60:102734
PubMed 35247836
DOI 10.1016/j.scr.2022.102734

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