Generation of a heterozygous FUS-Q290X knock in human embryonic stem cell line (WAe009-A-83) using CRISPR/Cas9 system
Summary
Fused in Sarcoma (FUS) gene encodes FUS RNA binding protein, a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein complex, which is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm, and it has been implicated in regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. FUS gene mutations result in amyotrophic lateral sclerosis and Liposarcoma. This heterozygous FUS-Q290X knock in hESC line will be a valuable tool to investigate the disease mechanisms of amyotrophic lateral sclerosis and Liposarcoma. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang Y, Zhu J, Dai Y, Wang L, Liu R, Guo X |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102734 |
| PubMed | 35247836 |
| DOI | 10.1016/j.scr.2022.102734 |