Generation of a KCNQ1 (c.1032 + 2 T > C) mutant human embryonic stem cell line via CRISPR base editing
Summary
The KCNQ1 gene encodes a voltage-gated potassium channel, which plays an important role in the repolarization of myocardial action potentials. Mutations in this gene often result in type 1 long QT syndrome (LQT1). Here, we generated a KCNQ1 (c.1032 + 2 T > C) mutant human embryonic stem cell line (WAe009-A-1D) based on the transient expression adenine base editing system that converts base A to G. The WAe009-A-1D cell maintains the morphology, pluripotency, and normal karyotype of the stem cells and is capable of differentiating into all three germ layers in vivo. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Jiang X, Fu C, Liu Q, Gao J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103425 |
| PubMed | 38653148 |
| DOI | 10.1016/j.scr.2024.103425 |