hPSCreg®
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WAe009-A-O

Registration Summary:

CRX-GFP WAe009

WAe009-A-O

General

Cell Line
hPSCreg name WAe009-A-O
Cite as:
WAe009-A-O (RRID:CVCL_VP31)
Alternative name(s)
CRX-GFP WAe009
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
WAe009-A-72
(COL1A2 -/-)
Last update 27th April 2023
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Provider
Generator University of Newcastle (UNEW)
Owner University of Newcastle (UNEW)
Distributors
Derivation country United Kingdom

External Databases
BioSamples SAMEA112970166
Cellosaurus CVCL_VP31
Wikidata Q93937313

General Information
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)
Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Versene
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
–
Differentiation Potency

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46 XX
Collin_et_al_Suppl__Figure_3_copy.tif
Passage number: 50
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Gene knock-in
19q13.33
19q13.33
ZFNs designed to cleave in the 3’ UTR of CRX were transfected into hESCs along with a donor construct containing homology to the target region, eGFP reporter and a puromycin selection cassette. Following selection, PCR and sequencing analysis of antibiotic resistant clones indicated targeted integration of the reporter cassette at the 3’ of the CRX gene, generating a CRX-GFP fusion. Further analysis of a clone exhibiting homozygote integration of the GFP reporter was conducted suggesting genomic stability was preserved and no other copies of the targeting cassette were inserted elsewhere within the genome.
Homologous Recombination