EBNA1+H9, H9-EBNA
WAe009-A-10
General
Cell Line |
|
| hPSCreg name | WAe009-A-10 |
| Cite as: | WAe009-A-10 (RRID:CVCL_C100) |
| Alternative name(s) |
EBNA1+H9, H9-EBNA
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-77 (H9-GFP) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1V (H9-AAVS1-Teton-KrasG12D) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Central South University (CSU)
Contact:
Cancer Research Institute |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA104620317 |
| Cellosaurus | CVCL_C100 |
| Wikidata | Q54993563 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Alternatives to consent |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex | |||||||||
| Feeder cells |
Mouse Embryonic Fibroblast Cellfinder Ont Id: EFO_0004040 |
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| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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