WAe009-A-10

EBNA1+H9, H9-EBNA

General#

Cell Line

hPSCreg Name WAe009-A-10
Alternative name(s)
EBNA1+H9, H9-EBNA
Cell line type Human embryonic stem cell (hESC)
Last update 21st November 2017
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Provider

Generator Central South University (CSU)
Derivation country China

External Databases

BioSamples SAMEA104620317
Cellosaurus CVCL_C100

General Information

Publications
Is the cell line available in principle?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA7768918

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells Mouse Embryonic Fibroblast
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
hrbFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unkown

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
EBNA1 (target)
Transgene expression