WAe009-A-93

NIS-EGFP-Fluc H9

General

Cell Line

hPSCreg Name
WAe009-A-93
Alternative name(s)
NIS-EGFP-Fluc H9
Cell line type Human embryonic stem cell (hESC)
Last update 4th September 2022
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Provider

Generator National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW)
Owner National Center for Cardiovascular Diseases & Fuwai Hospital (NCCDFW)
Derivation country China

External Databases

BioSamples SAMEA110401623
CLO CLO_0104043

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
SLC5A5 (target)
Transgene expression
Nuclear molecular imaging reporter gene
Transposon
PiggyBack transposon
WB-NIS and Firefly luciferase expression.jpg
NIS and Firefly luciferase expression detected by Western-blotting
EGFP (target)
Transgene expression
fluorescence imaging reporter
Transposon
PiggyBack transposon
GFP.jpg
High expression of EGFP expression by confocal microscopy.
Firefly luciferase (target)
Transgene expression
bioluminescence imaging reporter,
Transposon
PiggyBack transposon