Generation of a NIS-EGFP-Fluc triple-reporter human embryonic stem cell line by PiggyBac transposon system
Summary
One major challenge in stem cell therapy is to longitudinally track cell fate after cells transplantation. Molecular Imaging approaches enabling noninvasive long-term monitoring the transplanted cells are imperative for assessment of the safety and efficiency. Here, we used PiggyBac technology to insert triple reporter genes: NIS, EGFP and Firefly luciferase into a human embryonic stem cell line (hESCs, H9) and obtained a reporter hESCs line (NIS-EGFP-Fluc H9). The triple-reporters allows the transplanted NIS-EGFP-Fluc H9 cells and their derivates to be fluorescence, bioluminescence and even PET/SPECT imaged. This triple-reporter hESCs line provides a valuable imaging platform for cell-based therapeutics clinical translation. Copyright © 2022. Published by Elsevier B.V.
| Authors | Huang P, Tian C, Chen Y, Zhou M, Ni J, Niu M, Liu X, Peng Z |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Dec;65:102968 |
| PubMed | 36403548 |
| DOI | 10.1016/j.scr.2022.102968 |