Generation of a TRPV1 knockout human pluripotent stem cell line (WAe009-A-U) using CRISPR/Cas9
Summary
The transient receptor potential vanilloid subfamily 1 (TRPV1) is a polymodal nociceptor that is highly expressed in sensory nerves. Activation of TRPV1 receptors excites primary afferent nociceptors by opening cation channels, allowing the influx of Na+ and Ca2+ ions into the cytoplasm. Here, a TRPV1 knockout human embryonic stem cell line was generated using the CRISPR/Cas9 genome-editing technology to further study the function of TRPV1. The cell line confirmed with normal pluripotency and karyotype. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Cao Y, Tang L, Su J, Wang H, Liang P, Rong K, Gong T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Oct;72:103202 |
| PubMed | 37708613 |
| DOI | 10.1016/j.scr.2023.103202 |