Generation of a TRPV1 knockout human pluripotent stem cell line (WAe009-A-U) using CRISPR/Cas9


The transient receptor potential vanilloid subfamily 1 (TRPV1) is a polymodal nociceptor that is highly expressed in sensory nerves. Activation of TRPV1 receptors excites primary afferent nociceptors by opening cation channels, allowing the influx of Na+ and Ca2+ ions into the cytoplasm. Here, a TRPV1 knockout human embryonic stem cell line was generated using the CRISPR/Cas9 genome-editing technology to further study the function of TRPV1. The cell line confirmed with normal pluripotency and karyotype. Copyright © 2023. Published by Elsevier B.V.

Authors Cao Y, Tang L, Su J, Wang H, Liang P, Rong K, Gong T
Journal Stem cell research
Publication Date 2023 Sep 9;72:103202
PubMed 37708613
DOI 10.1016/j.scr.2023.103202

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