General

Cell Line

hPSCreg name WAe009-A-Q
Cite as:
WAe009-A-Q (RRID:CVCL_C9J8)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
WAe009-A-72
(COL1A2 -/-)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
Last update 1st July 2024
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Provider

Generator St. Anna Kinderkrebsforschung GmbH (CCRI)
Owner St. Anna Kinderkrebsforschung GmbH (CCRI)
Derivation country Austria

External Databases

BioSamples SAMEA113610264
Cellosaurus CVCL_C9J8
Wikidata Q123033865

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution Contrains are listed in the MTA provided

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Other medium:
Base medium: Gibco StemFlex Medium
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • ES
  • Ewing's Sarcoma
  • Ewing Sarcoma
  • Ewing sarcoma
  • Ewing's Tumor
  • Ewing tumor
show more synonyms
Genetic modifications
EWSR1/FLI1 (target)
Gene knock-in
AAVS1 locus
TALEN