Generation of an S100B homozygous knockout pluripotent stem cell line (WAe009-A-94) by the CRISPR/Cas9 system
Summary
S100 calcium binding protein beta (S100B) is an S-100 low molecular weight binding protein that regulates intracellular processes. This protein is involved in myocardial contractility and calcium handling capacity. In this study, a human embryonic stem cell (hESC) line with homozygous S100B knockout (S100B-KO) was generated using the CRISPR/Cas9 editing system. This S100B-KO hESC line maintained normal cell morphology and karyotype, expressed pluripotency markers, and could differentiate into cells of all three germ layers. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang P, Wang H, Li S, Guo T, Zhang R, Zhou P, Ding S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Oct;64:102924 |
| PubMed | 36182708 |
| DOI | 10.1016/j.scr.2022.102924 |