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WAe009-A-96

Registration Summary:

hTRF1-KO #6

WAe009-A-96

General

Cell Line
hPSCreg name WAe009-A-96
Cite as:
WAe009-A-96 (RRID:CVCL_C3LI)
Alternative name(s)
hTRF1-KO #6
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A-95
(hTRF1-KO #3)
WAe009-A-97
(hTRF1-KO #9)
WAe009-A
(WA09, H9)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
WAe009-A-72
(COL1A2 -/-)
Last update 24th August 2022
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Provider
Generator Korea Research Institute of Bioscience and Biotechnology (KRIBB)

External Databases
BioSamples SAMEA110691899
Cellosaurus CVCL_C3LI
Wikidata Q114313219

General Information
Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)
Has the donor karyotype been analysed?
Yes
46xx
Karyotyping method: G-Banding

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells Mouse embryonic fibroblast cells
Passage method Enzyme-free cell dissociation
ReLeSR (Stem Cell technology)
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
LIN28A
Yes
Marker Present Absent
mCpG X
OCT4 X
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
NKX2.1
Yes
GATA4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
Vimentin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Nestin
Yes
Tuj1
Yes

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46xx
Passage number: p46
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
None
Synonyms
  • None at all
  • NONE
  • None
  • No abnormalities related to cardiovascular diseases
show more synonyms
Genetic modifications
TERF1 (target)
Gene knock-out
8q21.11
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
TERF1 (target)
Gene knock-out
8q21.11
CRISPR-associated (CRISPR/Cas) System