hTRF1-KO #6
WAe009-A-96
General
Cell Line |
|
| hPSCreg name | WAe009-A-96 |
| Cite as: | WAe009-A-96 (RRID:CVCL_C3LI) |
| Alternative name(s) |
hTRF1-KO #6
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A-95 (hTRF1-KO #3) WAe009-A-97 (hTRF1-KO #9) WAe009-A (WA09, H9) WAe009-A-77 (H9-GFP) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1V (H9-AAVS1-Teton-KrasG12D) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) |
| Last update | 24th August 2022 |
| User feedback | |
Provider |
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| Generator | Korea Research Institute of Bioscience and Biotechnology (KRIBB) |
External Databases |
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| BioSamples | SAMEA110691899 |
| Cellosaurus | CVCL_C3LI |
| Wikidata | Q114313219 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46xx
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
Mouse embryonic fibroblast cells |
| Passage method |
Enzyme-free cell dissociation
ReLeSR (Stem Cell technology)
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| LIN28A |
Yes |
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| Marker | Present | Absent |
| mCpG | X | |
| OCT4 | X |
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46xx
Passage number: p46
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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