hTRF1-KO #9
WAe009-A-97
General
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | NO |
Is the medical history available upon request? | NO |
Is clinical information available? | NO |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
Is there an MTA available for the cell line? | Yes |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
Mouse embryonic fibroblasts |
Passage method |
Enzyme-free cell dissociation
ReLeSR (Stem Cell technology)
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CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
LIN28A |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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Alkaline Phosphatase |
Yes |
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Marker | Present | Absent |
mCpG | X | |
OCT4 | X |
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
In vitro spontaneous differentiation
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46 XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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Genetic modifications not related to a disease |
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