hTRF1-KO #3
WAe009-A-95
General
Cell Line |
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| hPSCreg name | WAe009-A-95 |
| Cite as: | WAe009-A-95 (RRID:CVCL_C3LH) |
| Alternative name(s) |
hTRF1-KO #3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A-96 (hTRF1-KO #6) WAe009-A-97 (hTRF1-KO #9) WAe009-A (WA09, H9) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-2D (TG.(ALK WT-GFP)) WAe009-A-2E (TG.(ALK F1174L-GFP)) WAe009-A-3L (H9-idCK-mC) WAe009-A-3M (H9-dCK-mC) WAe009-A-3N (H9-dCk) WAe009-A-58 (COL4A5 heterozygote) WAe009-A-1P (IsoHD-30Q-NGN2) WAe009-A-59 (dHAND KO) WAe009-A-1Q (IsoHD-65Q-NGN2) WAe009-A-1R (IsoHD-81Q-NGN2) WAe009-A-1W (H9 EF1A-mCherry-noPuro 803_AAVS-iSHH #3) WAe009-A-60 (H9 iNK5A) |
| Last update | 24th August 2022 |
| User feedback | |
Provider |
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| Generator | Korea Research Institute of Bioscience and Biotechnology (KRIBB) |
External Databases |
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| BioSamples | SAMEA110666770 |
| Cellosaurus | CVCL_C3LH |
| Wikidata | Q114313218 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | Yes |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| NANOG |
Yes |
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| Alkaline Phosphatase |
Yes |
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| Marker | Present | Absent |
| mCpG | X | |
| OCT4 | X |
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46xx
Passage number: 45
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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