Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing


Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Xie L, Huang J, Li X, Dai L, Lin X, Zhang J, Luo J, Zhang W
Journal Stem cell research
Publication Date 2019 Dec;41:101610
PubMed 31775087
DOI 10.1016/j.scr.2019.101610

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