Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system

Summary

S100A1 is a calcium-binding protein involved in myocardial contractility,which possesses a high affinity for calcium.  Several studies have demonstrated that S100A1 is a protector against myocardial injury. In this study, we have generated a homozygous S100A1 knockout (S100A1-KO) human embryonic stem cell (hESC) line by the CRISPR/Cas9 editing system. This S100A1-KO hESC line maintained normal morphology, pluripotency and karyotype, which can differentiate into three germ layers in vivo. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang S, Guo T, Song Y, Jiang M, Jiang Y, Dong T, Zhang S, Wang H, Hou X
Journal Stem cell research
Publication Date 2021 Dec 20;59:102631
PubMed 34953326
DOI 10.1016/j.scr.2021.102631

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