CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure


The role of muscle LIM protein (MLP), encoded by CSRP3, is not well understood in humans. CSRP3 knockout mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Using CRISPR/Cas9, we generated an MLP deficient human ESC line WAe009-A-41 carrying a compound heterozygous 13 bp deletion/1 bp insertion in the CSRP3 gene. The WAe009-A-41 line exhibited a normal female karyotype (46, XX), expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. MLP expression was not detectable in WAe009-A-41 line. This cell line can be a useful tool for studying the role of CSRP3 in cardiomyopathy and heart failure. Copyright © 2020. Published by Elsevier B.V.

Authors Sun L, Li J, Li E, Niu S, Qin Z, Zhi Q, Zhao J, Xiong H, Li Y, Jian L, Zhang L
Journal Stem cell research
Publication Date 2020 Dec;49:102077
PubMed 33176267
DOI 10.1016/j.scr.2020.102077

Research Projects

Cell Lines