Generation of a BEST1 Pr-EGFP reporter human embryonic stem cell line via CRISPR/Cas9 editing
Summary
The retinal pigment epithelium (RPE) cell, located between the neural retina and choriocapillaris, is vital for retinal maintenance and photoreceptor function. Human embryonic stem cells (hESCs) provide a limitless source of RPE cells for transplantation. Using CRISPR/Cas9, we inserted a fusion of the BEST1 promoter (an RPE-specific marker) and the EGFP gene into the AAVS1 locus to track differentiation in hESC-induced RPE (hESC-iRPE). The resulting gene-edited line, WAe009-A-2 M, maintained a normal karyotype, expressed pluripotency markers, and demonstrated differentiation potential, making it invaluable for RPE development and therapeutic research. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Lu S, Chen M, Liu X, Li J, Liu H, Li S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec 9;82:103625 |
| PubMed | 39667064 |
| DOI | 10.1016/j.scr.2024.103625 |