AGK-KO C10
The cell line is not validated yet.
WAe009-A-3C
General
Cell Line |
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| hPSCreg name | WAe009-A-3C |
| Cite as: | WAe009-A-3C |
| Alternative name(s) |
AGK-KO C10
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 24th October 2025 |
| User feedback | |
Provider |
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| Generator | Murdoch Children's Research Institute (MCRI) |
| Owner | Murdoch Children's Research Institute (MCRI) |
| Distributors | |
| Derivation country | Australia |
External Databases |
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| BioSamples | SAMEA118422836 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
| Surface coating | Non coated | ||||||||||||
| Feeder cells |
MEF |
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| Passage method |
Enzymatically
TrypLE
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| O2 Concentration | 5 % | ||||||||||||
| CO2 Concentration | 5 % | ||||||||||||
| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT4) |
Yes |
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| SSEA-4 |
Yes |
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| NANOG |
Yes |
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| TRA 1-81 |
Yes |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XX
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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