H9.1, WA09.1
WAe009-A-1
General
Cell Line |
|
| hPSCreg name | WAe009-A-1 |
| Cite as: | WAe009-A-1 (RRID:CVCL_C811) |
| Alternative name(s) |
H9.1, WA09.1
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-77 (H9-GFP) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1V (H9-AAVS1-Teton-KrasG12D) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) |
| Last update | 22nd March 2022 |
| User feedback | |
Provider |
|
| Generator | WiCell Research Institute (WA) |
| Derivation country | United States |
External Databases |
|
| Cellosaurus | CVCL_C811 |
| Wikidata | Q54993561 |
| BioSamples | SAMEA13600231 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Telomerase Activity |
Yes |
|
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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