Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique


Serine hydroxymethyltransferase 2 (SHMT2), a catalytic enzyme playing an important role in aerobic cellular respiration and mitochondrial metabolism, might be pivotal in self-renewal and differentiation of human pluripotent stem cells. Herein, we used the CRISPR/Cas9 editing system to construct a homozygous SHMT2 knockout (SHMT2-KO) human embryonic stem cell (hESC) line, exhibiting a normal karyotype, colony morphology, and high expression levels of pluripotent proteins. Furthermore, SHMT2 knockout did not impact the self-renewal ability or differentiation potential into three germ layers of hESCs. Accordingly, this cell line provides a valuable model for further assessing SHMT2 functions in human embryonic development. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Li J, Zhang B, Gan M, Li Y, He L, Yue W, Qiao H, Pei X, Li Y
Journal Stem cell research
Publication Date 2021 Oct 19;57:102581
PubMed 34688993
DOI 10.1016/j.scr.2021.102581

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