Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique
Summary
Serine hydroxymethyltransferase 2 (SHMT2), a catalytic enzyme playing an important role in aerobic cellular respiration and mitochondrial metabolism, might be pivotal in self-renewal and differentiation of human pluripotent stem cells. Herein, we used the CRISPR/Cas9 editing system to construct a homozygous SHMT2 knockout (SHMT2-KO) human embryonic stem cell (hESC) line, exhibiting a normal karyotype, colony morphology, and high expression levels of pluripotent proteins. Furthermore, SHMT2 knockout did not impact the self-renewal ability or differentiation potential into three germ layers of hESCs. Accordingly, this cell line provides a valuable model for further assessing SHMT2 functions in human embryonic development. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Li J, Zhang B, Gan M, Li Y, He L, Yue W, Qiao H, Pei X, Li Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Dec;57:102581 |
| PubMed | 34688993 |
| DOI | 10.1016/j.scr.2021.102581 |