Generation of a homozygous CPAMD8 knockout human embryonic stem cell line (WAe009-A-2R) by CRISPR/Cas9 system
Summary
CPAMD8, a constituent of the A2M/C3 (α-2-macroglobulin/complement 3) protein family, is strikingly expressed in the human fetal lens and distal neural retina. Mutations in CPAMD8 have been linked to anterior segment dysgenesis and primary congenital glaucoma. We utilized CRISPR/Cas9 technology to establish a homozygous CPAMD8 knockout human embryonic stem cell line for differentiating retinal organoids, with the intent of exploring the role of CPAMD8 in the early development of the human eye. The CPAMD8 knockout cell line exhibits normal morphology, pluripotency, and karyotype, serving as a valuable research tool for investigating the functions of CPAMD8 in ophthalmology. Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Pan W, Yang Y, Zhang S, Liu X, Hu H, Qu J, Liu H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Apr;84:103683 |
| PubMed | 39978205 |
| DOI | 10.1016/j.scr.2025.103683 |