WAe009-A-2R

General

Cell Line

hPSCreg name WAe009-A-2R
Cite as:
WAe009-A-2R
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 19th January 2025
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Provider

Generator Institute of Biomedical Big Data(IBBD) (IBBD)
Owner Institute of Biomedical Big Data(IBBD) (IBBD)
Derivation country China

External Databases

BioSamples SAMEA117605522

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • ASGD8
  • CPAMD8 anterior segment dysgenesis
  • CPAMD8-related anterior segment dysgenesis
  • anterior segment dysgenesis 8
  • anterior segment dysgenesis caused by mutation in CPAMD8
  • anterior segment dysgenesis type 8
show more synonyms
Genetic modifications
CPAMD8 (target)
Gene knock-out
19p13.11
CRISPR-associated (CRISPR/Cas) System